Human Prorenin Direct ELISA Kit, IHPRENKT
The sensitive measurement of human prorenin is easily
performed with this 96 well strip format ELISA kit. Active renin is not
detected by this assay. Prorenin is measured directly by ELISA without pretreatment
of samples or conversion to renin. Human prorenin in plasma, serum, or cell
culture media binds capture antibody coated onto a micro titer plate. Anti-human
prorenin primary antibody binds to the captured prorenin. Excess antibody is
washed away, and bound antibody is then reacted with the secondary antibody.
TMB substrate is used for color development at 450 nm. All reagents and
standards are provided in these ELISA kits. This kit does not cross react with
mouse or rat prorenin.
Mouse monoclonal antibody directed to human prorenin. This antibody recognizes and binds only to Prorenin. It does not bind or otherwise interact with active Renin. No cross reactivity with mouse or rat prorenin.
IgG2b class. Suitable for: WB, ELISA. Also available Biotin and FITC labelled.
This monoclonal antibody is inhibitory (Blocks proteolytic conversion to renin). Unpublished data demonstrates that this monoclonal antibody blocks activation of prorenin to renin after the addition of catalytic trypsin.
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